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Brucellosis is a major bacterial zoonosis involving many mammals, including domestic cows (Brucella abortus), pigs (B. suis), goats/sheep (B. melitensis), and dogs (B. canis). The disease is also found in wild ruminant mammals such as deer, elk and moose.
Tests generally appear in the order most useful for common clinical situations
| Test name: CBC with Platelet Count & Automated Differential |
| ARUP #: 0040003 |
| Methodology: Automated Cell Count with Flow Cell Differential |
| Use: May be helpful in differentiating bacterial from viral etiology |
| Test name: Sedimentation Rate, Westergren (ESR) |
| ARUP #: 0040325 |
| Methodology: Westergren |
| Use: Determine if elevated as an indicator of endocarditis |
| Test name: Hepatic Function Panel |
| ARUP #: 0020416 |
| Methodology: Refer to individual components. |
| Use: Test for presence of hepatitis Panel includes albumin, alkaline phosphatase, aspartate aminotransferase, bilirubin direct, protein total, bilirubin total |
| Test name: Brucella Culture |
| ARUP #: 0060159 |
| Methodology: Standard reference procedures for Brucella culture and identification |
| Use: Gold standard for detection of Brucella in blood (optimum), CSF, body fluids and abscesses |
| Limitations: Time intensive Because isolation of organism is difficult, serologic tests are generally used for diagnosis |
| Test name: Brucella Antibodies, IgG & IgM by ELISA and Bacterial Agglutination |
| ARUP #: 2002853 |
| Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Bacterial Agglutination |
| Use: Detect presence of Brucella antibodies in serum samples from patients with congruent clinical history |
| Limitations: Cross reactions may occur between Brucella and Francisella tularensis antigens and antisera; parallel tests should be run with these antigens |
| Follow-up: CDC recommends that specimens testing positive or equivocal for Brucella antibodies by ELISA be confirmed by bacterial agglutination; best evidence for current infection is significant change on two appropriately timed specimens done in the same laboratory at the same time |
| Test name: Brucella Antibody (Total) by Agglutination |
| ARUP #: 0050135 |
| Methodology: Semi-Quantitative Bacterial Agglutination |
| Use: Detect presence of Brucella antibodies in serum samples from patients with congruent clinical history |
| Limitations: Cross-reactions may occur between Brucella and Francisella tularensis antigens and antisera; parallel tests should be run with these antigens |
| Test name: Organism Identification by 16S rDNA Sequencing |
| ARUP #: 0060720 |
| Methodology: 16S rDNA Sequencing |
| Comments: |
| Test name: Aerobic Organism Identification |
| ARUP #: 0060115 |
| Methodology: Identification methods including Biochemical, MALDI TOF and/or 16S rDNA sequencing. |
| Comments: Include warning if organism suspected to be dangerous pathogen (eg, Salmonella, Shigella, Neisseria meningitides, etc) For suspected agents of bioterrorism, notify state department of health and refer isolates to state laboratory for identification Susceptibilities on agents of bioterrorism are not performed at ARUP |
| Test name: Febrile Antibodies Panel |
| ARUP #: 2001789 |
| Methodology: Semi-Quantitative Direct Agglutination/Semi-Quantitative Indirect Fluorescent Assay/Qualitative ImmunoDOT |
| Comments: |