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Bartonella spp cause several different diseases, including cat scratch disease (CSD).
Tests generally appear in the order most useful for common clinical situations
| Test name: CBC with Platelet Count & Automated Differential |
| ARUP #: 0040003 |
| Methodology: Automated Cell Count with Flow Cell Differential |
| Use: May help differentiate between bacterial and viral etiology |
| Test name: Bartonella DNA Detection by PCR |
| ARUP #: 0093057 |
| Methodology: Qualitative Polymerase Chain Reaction |
| Use: Rapid test for serum, plasma, CSF or tissue In epidemiological situations, determine Bartonella species (sequencing of PCR product) |
| Limitations: Negative result does not rule out the presence of Bartonella spp DNA in quantities below the sensitivity of assay or the possibility of PCR inhibitors in samples Unidentified sequence variations may lead to a false-negative result |
| Test name: Bartonella DNA Detection by PCR, Whole Blood |
| ARUP #: 0060762 |
| Methodology: Qualitative Polymerase Chain Reaction |
| Use: Rapid test for whole blood In epidemiological situations, determine Bartonella species (sequencing of PCR product) |
| Limitations: Negative result does not rule out the presence of Bartonella spp DNA in quantities below the sensitivity of assay or the possibility of PCR inhibitors in samples Unidentified sequence variations may lead to a false-negative result |
| Test name: Bartonella henselae & B. quintana Antibodies, IgG & IgM |
| ARUP #: 2002280 |
| Methodology: Semi-Quantitative Indirect Fluorescent Antibody |
| Use: Order when current or past exposure to B. henselae or B. quintana suspected Panel includes B. henselae antibody and B. quintana antibody Confirmation requires a fourfold change in titers between acute and convalescent specimens |
| Limitations: Low positive IgG result suggests past exposure or infection; high positive result may indicate current or recent infection but not conclusively Antibodies take up to 10 days to develop While IgM antibodies suggest recent infection, low levels may occasionally persist >12 months post-infection Best evidence of infection is significant change on 2 appropriately timed specimens, where both tests are performed in same laboratory at same time |
| Follow-up: If test results are equivocal, repeat testing in 10-14 days |
| Test name: Blood Culture |
| ARUP #: 0060102 |
| Methodology: Continuous Monitoring Blood Culture/Identification |
| Use: Use in patients with endocarditis to determine presence of Bartonella species |
| Limitations: Lysis-centrifugation may also be used PCR may be more sensitive Testing is limited to the University of Utah Health Sciences Center only |
| Test name: Histochemical Stains |
| ARUP #: |
| Methodology: |
| Use: Available stains include Spirochetes and Legionella (Steiner & Steiner method) |
| Test name: Bartonella henselae (Cat Scratch) Antibodies, IgG & IgM by IFA |
| ARUP #: 0050108 |
| Methodology: Semi-Quantitative Indirect Fluorescent Antibody |
| Comments: May take up to 10 days for antibodies to develop; titers may be elevated for years |
| Test name: Bartonella quintana Antibodies, IgG & IgM by IFA |
| ARUP #: 0050106 |
| Methodology: Semi-Quantitative Indirect Fluorescent Antibody |
| Comments: May take up to 10 days for antibodies to develop; titers may be elevated for years |
| Test name: Bartonella henselae Antibody, IgG by IFA |
| ARUP #: 0050091 |
| Methodology: Semi-Quantitative Indirect Fluorescent Antibody |
| Comments: |
| Test name: Bartonella henselae Antibody, IgM by IFA |
| ARUP #: 0050092 |
| Methodology: Semi-Quantitative Indirect Fluorescent Antibody |
| Comments: |
| Test name: Bartonella quintana Antibody, IgG by IFA |
| ARUP #: 0050094 |
| Methodology: Semi-Quantitative Indirect Fluorescent Antibody |
| Comments: |
| Test name: Bartonella quintana Antibody, IgM by IFA |
| ARUP #: 0050093 |
| Methodology: Semi-Quantitative Indirect Fluorescent Antibody |
| Comments: |